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KMID : 1094720140190020257
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 2 p.257 ~ p.261
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Label-free measurement of cell viability via counting cells attached on affinity substrates
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Ahn Jun-Hyoung
Park Jin-A
Kim Yeon-Gu
Lee Eun-Gyo
Kim Min-Gon
Shin Yong-Beom
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Abstract
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The commonly used trypan blue dye exclusion method and other modified cell viability methods, such as fluorescein dye and tetrazolium dye exclusion, artificially introduce toxic chemicals to cells and, thus, alter cellular organelles when measuring cell viability. Therefore, cell viability could be affected by the processes currently used to observe viability. In this study, the cell viability of Chinese hamster ovary (CHO) cells was measured by simply counting attached cells after the cultured CHO cells were attached on a Concanavalin A (Con A) substrate. The efficiency of cell attachment to Con A surfaces was different for live and dead cells allowing the cell viability of CHO cells to be measured without any chemical modifications to the cells.
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KEYWORD
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cell viability, label-free measurement, cell attachment, CHO cell, affinity binding
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